中国农科院蔬菜花卉研究所2017年“青年英才计划”人才招聘公告

Tomato yellow leaf curl virus differentially influences plant defense responses to a vector and a no

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Qi Su,Mark C. Mescher,Shaoli Wang,Gong Chen,Wen Xie,Qingjun Wu,Wenkai Wang,Youjun Zhang

Accepted manuscript online: 5 October 2015 Full publication history
DOI: 10.1111/pce.12650View/save citation
Cited by: 0 articles Check for new citations

This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: 10.1111/pce.12650.
ABSTRACT

Plants frequently engage in simultaneous interactions with diverse classes of biotic antagonists. Differential induction of plant defense pathways by these antagonists, and interactions between pathways, can have important ecological implications; however, these effects are currently not well understood. We explored how Tomato yellow leaf curl virus (TYLCV) influenced the performance of its vector (Bemisia tabaci) and a non-vector herbivore (Tetranychus urticae) occurring separately or together on tomato plants (Solanum lycopersicum). TYLCV enhanced the performance of B. tabaci, though this effect was statistically significant only in the absence of T. urticae, which adversely affected B. tabaci performance regardless of infection status. In contrast, the performance of T. urticae was enhanced (only) by the combined presence of TYLCV and B. tabaci. Analyses of phytohormone levels and defense gene expression in wild-type tomatoes and various plant-defense mutants indicate that the enhancement of herbivore performance (for each species) entails the disruption of downstream defenses in the jasmonic acid (JA) pathway. For T. urticae, this disruption appears to involve antagonistic effects of salicylic acid (SA), which is cumulatively induced to high levels by B. tabaci and TYLCV. In contrast, TYLCV was found to suppress JA-mediated responses to B. tabaci via mechanisms independent of SA. This article is protected by copyright. All rights reserved.

论文链接: http://onlinelibrary.wiley.com/doi/10.1111/pce.12650/full

              

蔬菜虫害防控创新团队揭示昆虫—病毒—寄主植物互作关系

    由我所张友军研究员领衔的蔬菜虫害防控创新团队利用生物学、生态学和分子生物学方法并结合突变体材料的应用,发现植物诱导产生的防御途径可通过相互间的“通话”调控不同有害生物种群的暴发。相关研究结果“Tomato yellow leaf curl virus differentially influences plant defense responses to a vector and a non-vector herbivore”(番茄黄化曲叶病毒与载体和非载体昆虫的组合影响寄主植物防御反应并进而影响有害生物) 于近日在线发表在国际著名期刊《Plant Cell & Environment》(《植物细胞与环境》)上。
    据悉,植物-昆虫-病原物在长期的进化过程中形成了复杂的互作关系。昆虫和病原物危害寄主植物,寄主植物则通过诱导产生的防御机制来抵御这种危害。植物产生的防御机制主要有茉莉酸(JA)途径、水杨酸(SA)途径和乙烯(ET)途径等。通常来说,茉莉酸途径主要由昆虫取食植物组织激活而形成,而水杨酸途径主要由病原物侵染植物组织激活。在自然环境中,植物往往同时受到昆虫和病原物的共同为害,这可能导致植物激活不同的防御途径,并在这些途径间形成“通话”效应。
    烟粉虱是一种世界性的重大农业害虫,可传播至少240种植物病毒病。番茄黄化曲叶病毒(TYLCV)是一类由烟粉虱传播的单链环状植物病毒。二斑叶螨则是危害番茄植物的常见螨类。蔬菜虫害防控创新团队研究发现,TYLCV与烟粉虱互作能显著地抑制番茄植物的JA防御反应,从而促进烟粉虱种群增长,但在二斑叶螨与TYLCV和烟粉虱共存的情况下,这种促进作用消失。相反,在二斑叶螨、TYLCV和烟粉虱三者共存的情况下却能显著地增加二斑叶螨在番茄植物上的产卵量。进一步利用JA和SA突变体番茄研究发现,造成这种生物学差异的原因在于不同的有害生物组合共同危害植株时导致了不同防御途径间的“通话”效应。在三者共存情况下下,抑制的JA效应受到诱导的SA途径的调控;而在仅有TYLCV与烟粉虱情况下,抑制的JA效应不受SA途径的调控。研究结果为揭示有害生物种群暴发的分子机制,针对性地开发有害生物防治方法有重要的理论与实际意义。
    该研究得到国家基金重点国际合作项目和中国农业科学院科技创新工程项目资助。

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