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论文题目: Dissecting the genome of the polyploid crop oilseed rape by transcriptome sequencing.
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发表年度: 2012-07-06 18:00:00.0
Bancroft, I., Morgan, C., Fraser, F., Higgins, J., Wells, R., Clissold, L., Baker, D., Long, Y., Meng, J., Xiaowu Wang, Liu, S., and Trick, M.

Nature Biotechnology,29,762-766,(2011)
DOI: 10.1038/nbt.1926

Abstract: Genetic linkage maps play a key role in marker-assisted predictive crop breeding by defining the genomic positions of trait-controlling loci. Relationships between the positions of such loci and genome sequences can be deduced, at least in principle, when sequence-based molecular markers are used to construct linkage maps. This enables the identification of candidate genes linked to phenotypes of interest. For plants with relatively simple genomes, high-throughput sequencing approaches permit the assembly of sequence scaffolds representing most of the total gene space, particularly when combined with conventional Sanger sequencing2, 3, 4, 5, 6, 7. However, genomes assembled by these methods are not as complete as the earliest genomes for model species8, 9. Moreover, the capacities of whole-genome approaches are particularly limited for species such as bread wheat and oilseed rape with recent polyploidy events in their ancestry, owing to the difficulty in correctly assembling very closely related sequences.

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